• Western blot analysis of extracts of mouse heart, using MCM10 antibody (STJ116280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MCM10 antibody (616-875) (STJ116280)

SKU:
STJ116280

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCM10 (616-875) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCM10
Gene ID: 55388
Uniprot ID: MCM10_HUMAN
Immunogen Region: 616-875
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 616-875 of human MCM10 (NP_877428.1).
Immunogen Sequence: RLEGAPATMTPKLGRGVLEG DDVLFYDESPPPRPKLSALA EAKKLAAITKLRAKGQVLTK TNPNSIKKKQKDPQDILEVK ERVEKNTMFSSQAEDELEPA RKKRREQLAYLESEEFQKIL KAKSKHTGILKEAEAEMQER YFEPLVKKEQMEEKMRNIRE VKCRVVTCKTCAYTHFKLLE TCVSEQHEYHWHDGVKRFFK CPCGNRSISLDRLPNKHCSN CGLYKWERDGMLKEKTGPK
Function Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability.acts as a direct target of transcriptional repression by ZBTB38.
Protein Name Protein Mcm10 Homolog
Hsmcm10
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Colocalizes With Orc2 In Nuclei Foci
Associated With Chromatin In S Phase
From Early To Mid-S Phase Located In Discrete Nuclear Foci
In Early S Phase
Several Hundred Foci Appeared Throughout The Nucleus
In Mid-S Phase
The Foci Appeared At The Nuclear Periphery And Nucleolar Regions
In The Late S And G Phases Localized To Nucleoli
Alternative Antibody Names Anti-Protein Mcm10 Homolog antibody
Anti-Hsmcm10 antibody
Anti-MCM10 antibody
Anti-PRO2249 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance