• Western blot analysis of T-47D, using MCCC1 rabbit polyclonal antibody (STJ112060) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates, using MCCC1 rabbit polyclonal antibody (STJ112060) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using MCCC1 rabbit polyclonal antibody (STJ112060) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using MCCC1 rabbit polyclonal antibody (STJ112060) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MCCC1 antibody (640-725) (STJ112060)

SKU:
STJ112060

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCCC1 (640-725) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCCC1
Gene ID: 56922
Uniprot ID: MCCA_HUMAN
Immunogen Region: 640-725
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 640-725 of human MCCC1 (NP_064551.3).
Immunogen Sequence: VSSQETQGGPLAPMTGTIEK VFVKAGDKVKAGDSLMVMIA MKMEHTIKSPKDGTVKKVFY REGAQANRHTPLVEFEEEES DKRESE
Post Translational Modifications Acetylated.
Function Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
Protein Name Methylcrotonoyl-Coa Carboxylase Subunit Alpha - Mitochondrial
Mccase Subunit Alpha
3-Methylcrotonyl-Coa Carboxylase 1
3-Methylcrotonyl-Coa Carboxylase Biotin-Containing Subunit
3-Methylcrotonyl-Coa -Carbon Dioxide Ligase Subunit Alpha
Database Links Reactome: R-HSA-196780
Reactome: R-HSA-3371599
Reactome: R-HSA-70895
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Methylcrotonoyl-Coa Carboxylase Subunit Alpha - Mitochondrial antibody
Anti-Mccase Subunit Alpha antibody
Anti-3-Methylcrotonyl-Coa Carboxylase 1 antibody
Anti-3-Methylcrotonyl-Coa Carboxylase Biotin-Containing Subunit antibody
Anti-3-Methylcrotonyl-Coa -Carbon Dioxide Ligase Subunit Alpha antibody
Anti-MCCC1 antibody
Anti-MCCA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance