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Anti-MC4R nanobody [SAA0796] (STJN000010)
General Information
| Short Description | Alpaca monoclonal nanobody anti-Melanocortin Receptor 4 is suitable for use in Universaling, ELISA and Flow Cytometry research applications. |
| Applications | Blocking/ELISA/FC |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA0796 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | MC4R |
| Gene ID | 4160 |
| Uniprot ID | MC4R_HUMAN |
Additional Info
| Function | Hormone receptor that acts as a key component of the leptin-melanocortin pathway at the intersection of homeostatic maintenance of energetic state. Plays a role in regulating food intake: activation by a stimulating hormone such as anorexigenic alpha-melanocyte stimulating hormone (alpha-MSH) inhibits appetite, whereas binding to a natural antagonist like Agouti-related protein/AGRP promotes appetite. G-protein-coupled receptor that activates conventional Galphas signaling leading to induction of anorexogenic signaling in the hypothalamus to result in negative energy balance. Regulates the firing activity of neurons from the hypothalamus by alpha-MSH and AGRP independently of Galphas signaling by ligand-induced coupling of closure of inwardly rectifying potassium channel KCNJ13. In intestinal epithelial cells, plays a role in the inhibition of hepatic glucose production via nesfatin-1/NUCB2 leading to increased cyclic adenosine monophosphate (cAMP) levels and glucagon-like peptide 1 (GLP-1) secretion in the intestinal epithelium. |
| Protein Name | Melanocortin Receptor 4Mc4-R |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-418555Reactome: R-HSA-9856649 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Nanobody Names | Anti-Melanocortin Receptor 4 nanobodyAnti-Mc4-R nanobodyAnti-MC4R nanobody |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Anti-MC4R nanobody [SAA0796] (STJN000010)
This nanobody binds Melanocortin 4 Receptor (MC4R), corresponding to UniProt P32245, with validated reactivity to human MC4R. It is raised in alpaca and is monoclonal, derived from clone SAA0796.
This nanobody has been validated for use in blocking assays, ELISA, and flow cytometry (FC). Researchers should optimise conditions for their specific experimental system, as dilution ranges are not specified in the current product data.
This nanobody is supplied in a VHH format carrying an 8His-Cys tag and is unconjugated. The His tag enables detection or capture via standard anti-His reagents or nickel-based pull-down approaches.
This nanobody is purified by nickel column affinity chromatography and is greater than 95% pure as determined by SDS-PAGE. It is supplied at 1 mg/mL in 0.01M PBS, pH 7.4.
For short-term use of 1 to 2 weeks, store at 4 degrees C. For storage up to 12 months, store at -20 degrees C, and for long-term storage, keep at -80 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
No dissociation constant or affinity constant data is currently provided in the product specifications for this nanobody. Researchers requiring binding kinetics data may wish to contact St John's Laboratory directly for any available characterisation information.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.