| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Mouse/Rat/Marmoset |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-MBP is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A dilution of 1: 1000 is recommended for IHC-P and 1:2000 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Mbp |
| Gene ID: |
17196 |
| Uniprot ID: |
MBP_MOUSE |
| Specificity: |
Specific for MBP. |
| Immunogen: |
A synthetic peptide from human MBP conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | As in other animals, several charge isomers may be produced as a result of optional post-translational modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. Methylated on arginine residues.decreases with the age of the animal, making MBP more cationic. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells. |
| Function | The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. |
| Protein Name | Myelin Basic ProteinMbpMyelin A1 Protein |
| Cellular Localisation | Isoform 13: Myelin MembranePeripheral Membrane ProteinCytoplasmic SideIsoform 12: Myelin MembraneIsoform 11: Myelin MembraneIsoform 10: Myelin MembraneIsoform 9: Myelin MembraneIsoform 8: Myelin MembraneIsoform 7: Myelin MembraneIsoform 6: Myelin MembraneIsoform 5: Myelin MembraneIsoform 4: Myelin MembraneIsoform 3: CytoplasmNucleusIsoform 2: CytoplasmIsoform 1: Cytoplasm |
| Alternative Antibody Names | Anti-Myelin Basic Protein antibodyAnti-Mbp antibodyAnti-Myelin A1 Protein antibodyAnti-Mbp antibodyAnti-Shi antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
