| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-MBD2-Isoform 1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in liter |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MBD2 |
| Gene ID: | 8932 |
| Uniprot ID: | MBD2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_003918.1 |
| Specificity: | This antibody is expected to resognize isoform 1 (NP_003918.1) only. |
| Immunogen Sequence: | RNDPLNQNKGKPDLN |
| Function | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
| Protein Name | Methyl-Cpg-Binding Domain Protein 2DemethylaseDmtaseMethyl-Cpg-Binding Protein Mbd2 |
| Database Links | Reactome: R-HSA-427413Reactome: R-HSA-73728 |
| Cellular Localisation | NucleusChromosomeNuclearIn Discrete FociDetected At Replication Foci In Late S PhaseLocalizes To Methylated ChromatinLocalizes To Sites Of Dna Damage In A Manner Partially Dependent On Zmynd8 |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Domain Protein 2 antibodyAnti-Demethylase antibodyAnti-Dmtase antibodyAnti-Methyl-Cpg-Binding Protein Mbd2 antibodyAnti-MBD2 antibody |
Information sourced from Uniprot.org