• Immunofluorescence analysis of A549 cells, using MARK4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MARK4 antibody (461-510 aa) (STJ94018)

SKU:
STJ94018

Current Stock:
Host: Rabbit
Applications: IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP/microtubule affinity-regulating kinase 4 (461-510 aa) is suitable for use in Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARK4
Gene ID: 57787
Uniprot ID: MARK4_HUMAN
Immunogen Region: 461-510 aa
Specificity: MARK4 Polyclonal Antibody detects endogenous levels of MARK4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MARK4 at the amino acid range 461-510
Post Translational Modifications Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X. Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylated throughout the cell cycle.
Function Serine/threonine-protein kinase. Phosphorylates the microtubule-associated protein MAPT/TAU. Also phosphorylates the microtubule-associated proteins MAP2 and MAP4. Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles. Required for the initiation of axoneme extension during cilium assembly. Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro. Plays a role in cell cycle progression, specifically in the G1/S checkpoint. Reduces neuronal cell survival. Plays a role in energy homeostasis by regulating satiety and metabolic rate. Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway. Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation. Involved in NLRP3 positioning along microtubules by mediating NLRP3 recruitment to microtubule organizing center (MTOC) upon inflammasome activation.
Protein Name Map/Microtubule Affinity-Regulating Kinase 4
Map/Microtubule Affinity-Regulating Kinase-Like 1
Database Links Reactome: R-HSA-5620912
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cilium Basal Body
Cilium Axoneme
Cell Projection
Dendrite
Localized At The Tips Of Neurite-Like Processes In Differentiated Neuroblast Cells
Detected In The Cytoplasm And Neuropil Of The Hippocampus
Alternative Antibody Names Anti-Map/Microtubule Affinity-Regulating Kinase 4 antibody
Anti-Map/Microtubule Affinity-Regulating Kinase-Like 1 antibody
Anti-MARK4 antibody
Anti-KIAA1860 antibody
Anti-MARKL1 antibody

Information sourced from Uniprot.org

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