| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MARK1 (560-700) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MARK1 |
| Gene ID: |
4139 |
| Uniprot ID: |
MARK1_HUMAN |
| Immunogen Region: |
560-700 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 560-700 of human MARK1 (NP_061120.3). |
| Immunogen Sequence: |
HPIKVTLPTIKDGSEAYRPG TTQRVPAASPSAHSISTATP DRTRFPRGSSSRSTFHGEQL RERRSVAYNGPPASPSHETG AFAHARRGTSTGIISKITSK FVRRDPSEGEASGRTDTSRS TSGEPKERDKEEGKDSKPRS L |
| Tissue Specificity | Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney. |
| Post Translational Modifications | Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity. Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity. |
| Function | Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). |
| Protein Name | Serine/Threonine-Protein Kinase Mark1Map/Microtubule Affinity-Regulating Kinase 1Par1 Homolog CPar-1cPar1c |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmCytoskeletonCell ProjectionDendriteAppears To Localize To An Intracellular Network |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Mark1 antibodyAnti-Map/Microtubule Affinity-Regulating Kinase 1 antibodyAnti-Par1 Homolog C antibodyAnti-Par-1c antibodyAnti-Par1c antibodyAnti-MARK1 antibodyAnti-KIAA1477 antibodyAnti-MARK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and USP4 knockout (KO) HeLa cells, using [KO Validated] USP4 Rabbit polyclonal antibody (STJ11100881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89815/358278/STJ11100881_1__46401.1713122625.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and USP4 knockout (KO) HeLa cells, using [KO Validated] USP4 Rabbit polyclonal antibody (STJ11100881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
