• Immunofluorescence analysis of U-2 OS cells using MARCKS Rabbit polyclonal antibody (STJ111047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using MARCKS Rabbit polyclonal antibody (STJ111047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using MARCKS Rabbit polyclonal antibody (STJ111047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from rat brain, using MARCKS Rabbit polyclonal antibody (STJ111047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MARCKS antibody (1-100) (STJ111047)

SKU:
STJ111047

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MARCKS (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARCKS
Gene ID: 4082
Uniprot ID: MARCS_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MARCKS (NP_002347.5).
Immunogen Sequence: MGAQFSKTAAKGEAAAERPG EAAVASSPSKANGQENGHVK VNGDASPAAAESGAKEELQA NGSAPAADKEEPAAAGSGAA SPSAAEKGEPAAAAAPEAGA
Post Translational Modifications Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
Function MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Protein Name Myristoylated Alanine-Rich C-Kinase Substrate
Marcks
Protein Kinase C Substrate - 80 Kda Protein - Light Chain
80k-L Protein
Pkcsl
Database Links Reactome: R-HSA-399997
Cellular Localisation Cytoplasm
Cytoskeleton
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Myristoylated Alanine-Rich C-Kinase Substrate antibody
Anti-Marcks antibody
Anti-Protein Kinase C Substrate - 80 Kda Protein - Light Chain antibody
Anti-80k-L Protein antibody
Anti-Pkcsl antibody
Anti-MARCKS antibody
Anti-MACS antibody
Anti-PRKCSL antibody

Information sourced from Uniprot.org

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