• Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night
  • Immunofluorescence analysis of Hela cell. 1, MARCH5 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-MARCHF5 antibody (21-70 aa) (STJ94011)

SKU:
STJ94011

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase MARCHF5 (21-70 aa) is suitable for use in Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARCHF5
Gene ID: 54708
Uniprot ID: MARH5_HUMAN
Immunogen Region: 21-70 aa
Specificity: MARCH5 Polyclonal Antibody detects endogenous levels of 40607 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MARCH5 at the amino acid range 21-70
Post Translational Modifications Autoubiquitinated leading to degradation (short half-life).
Function Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Protein Name E3 Ubiquitin-Protein Ligase Marchf5
Membrane-Associated Ring Finger Protein 5
Membrane-Associated Ring-Ch Protein V
March-V
Mitochondrial Ubiquitin Ligase
Mitol
Ring Finger Protein 153
Ring-Type E3 Ubiquitin Transferase Marchf5
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Authors Show That The Protein Can Be Detected In Endoplasmic Reticulum
Authors Show Its Presence Only In Mitochondria
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Marchf5 antibody
Anti-Membrane-Associated Ring Finger Protein 5 antibody
Anti-Membrane-Associated Ring-Ch Protein V antibody
Anti-March-V antibody
Anti-Mitochondrial Ubiquitin Ligase antibody
Anti-Mitol antibody
Anti-Ring Finger Protein 153 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Marchf5 antibody
Anti-MARCHF5 antibody
Anti-MARCH5 antibody
Anti-RNF153 antibody

Information sourced from Uniprot.org

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