• Western blot analysis of lysates from A549 and RAW264.7 cells, using MAPK9 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using JNK2 Polyclonal Antibody

Anti-MAPK9 antibody (246-295 aa) (STJ93807)

SKU:
STJ93807

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitogen-activated protein kinase 9 (246-295 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK9
Gene ID: 5601
Uniprot ID: MK09_HUMAN
Immunogen Region: 246-295 aa
Specificity: JNK2 Polyclonal Antibody detects endogenous levels of JNK2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MAPK9 at the amino acid range 246-295
Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation. Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation. MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
Protein Name Mitogen-Activated Protein Kinase 9
Map Kinase 9
Mapk 9
Jnk-55
Stress-Activated Protein Kinase 1a
Sapk1a
Stress-Activated Protein Kinase Jnk2
C-Jun N-Terminal Kinase 2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2871796
Reactome: R-HSA-450321
Reactome: R-HSA-450341
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Nucleus
Colocalizes With Pou5f1 In The Nucleus
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody
Anti-C-Jun N-Terminal Kinase 2 antibody
Anti-MAPK9 antibody
Anti-JNK2 antibody
Anti-PRKM9 antibody
Anti-SAPK1A antibody

Information sourced from Uniprot.org

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