Anti-MAPK6 antibody (372-721) (STJ117499)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MAPK6 (372-721) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAPK6 |
| Gene ID | 5597 |
| Uniprot ID | MK06_HUMAN |
| Immunogen Region | 372-721 |
| Immunogen Sequence | NFDIDEVQLDPRALSDVTDE EEVQVDPRKYLDGDREKYLE DPAFDTNYSTEPCWQYSDHH ENKYCDLECSHTCNYKTRSS SYLDNLVWRESEVNHYYEPK LIIDLSNWKEQSKEKSDKKG KSKCERNGLVKAQIALEEAS QQLAGKEREKNQGFDFDSFI AGTIQLSSQHEPTDVVDKLN DLNSSVSQLELKSLISKSVS QEKQEKGMANLAQLEALYQS SWDSQFVSGGEDCFFINQF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1). |
Additional Info
| Tissue Specificity | Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts. |
| Post Translational Modifications | Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway. |
| Function | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle. |
| Protein Name | Mitogen-Activated Protein Kinase 6Map Kinase 6Mapk 6Extracellular Signal-Regulated Kinase 3Erk-3Map Kinase Isoform P97P97-Mapk |
| Database Links | Reactome: R-HSA-5687128 |
| Cellular Localisation | CytoplasmNucleusTranslocates To The Cytoplasm Following Interaction With Mapkapk5 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 6 antibodyAnti-Map Kinase 6 antibodyAnti-Mapk 6 antibodyAnti-Extracellular Signal-Regulated Kinase 3 antibodyAnti-Erk-3 antibodyAnti-Map Kinase Isoform P97 antibodyAnti-P97-Mapk antibodyAnti-MAPK6 antibodyAnti-ERK3 antibodyAnti-PRKM6 antibody |
Information sourced from Uniprot.org