Anti-MAPK10 antibody (300-400) (STJ119530)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal JNK3 (300-400) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAPK10 |
| Gene ID | 5602 |
| Uniprot ID | MK10_HUMAN |
| Immunogen Region | 300-400 |
| Immunogen Sequence | NRPKYAGLTFPKLFPDSLFP ADSEHNKLKASQARDLLSKM LVIDPAKRISVDDALQHPYI NVWYDPAEVEAPPPQIYDKQ LDEREHTIEEWKELIYKEVM N |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human JNK3 (NP_620448.1). |
Additional Info
| Tissue Specificity | Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney. |
| Post Translational Modifications | Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro. Palmitoylation regulates subcellular location and axonal development. |
| Function | Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Also participates in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. |
| Protein Name | Mitogen-Activated Protein Kinase 10Map Kinase 10Mapk 10Map Kinase P49 3f12Stress-Activated Protein Kinase 1bSapk1bStress-Activated Protein Kinase Jnk3C-Jun N-Terminal Kinase 3 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-450341 |
| Cellular Localisation | CytoplasmMembraneLipid-AnchorNucleusMitochondrionPalmitoylation Regulates Mapk10 Trafficking To CytoskeletonRecruited To The Mitochondria In The Presence Of Sarm1 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 10 antibodyAnti-Map Kinase 10 antibodyAnti-Mapk 10 antibodyAnti-Map Kinase P49 3f12 antibodyAnti-Stress-Activated Protein Kinase 1b antibodyAnti-Sapk1b antibodyAnti-Stress-Activated Protein Kinase Jnk3 antibodyAnti-C-Jun N-Terminal Kinase 3 antibodyAnti-MAPK10 antibodyAnti-JNK3 antibodyAnti-JNK3A antibodyAnti-PRKM10 antibodyAnti-SAPK1B antibody |
Information sourced from Uniprot.org