• Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (STJ119100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using ERK1/ ERK2 rabbit polyclonal antibody (STJ119100) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using ERK1/ ERK2 rabbit polyclonal antibody (STJ119100) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using ERK1/ ERK2 rabbit polyclonal antibody (STJ119100) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using ERK1/ ERK2 rabbit polyclonal antibody (STJ119100) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ERK1/ ERK2 rabbit polyclonal antibody (STJ119100) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ERK1/ ERK2 rabbit polyclonal antibody (STJ119100) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using ERK1/ ERK2 rabbit polyclonal antibody (STJ119100) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119100)

SKU:
STJ119100

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Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK3
MAPK1
Gene ID: 5595
5594
Uniprot ID: MK03_HUMAN
MK01_HUMAN
Immunogen Region: 200-300
Specificity: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK1/ERK2 (NP_620407.1/NP_002737.2).
Immunogen Sequence: LNSKGYTKSIDIWSVGCILA EMLSNRPIFPGKHYLDQLNH ILGILGSPSQEDLNCIINLK ARNYLLSLPHKNKVPWNRLF PNADSKALDLLDKMLTFNPH K
Tissue Specificity
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 3 antibody
Anti-Map Kinase 3 antibody
Anti-Mapk 3 antibody
Anti-Ert2 antibody
Anti-Extracellular Signal-Regulated Kinase 1 antibody
Anti-Erk-1 antibody
Anti-Insulin-Stimulated Map2 Kinase antibody
Anti-Map Kinase Isoform P44 antibody
Anti-P44-Mapk antibody
Anti-Microtubule-Associated Protein 2 Kinase antibody
Anti-P44-Erk1 antibody
Anti-MAPK3 antibody
Anti-ERK1 antibody
Anti-PRKM3 antibody
Anti-Mitogen-Activated Protein Kinase 1 antibody
Anti-Map Kinase 1 antibody
Anti-Mapk 1 antibody
Anti-Ert1 antibody
Anti-Extracellular Signal-Regulated Kinase 2 antibody
Anti-Erk-2 antibody
Anti-Map Kinase Isoform P42 antibody
Anti-P42-Mapk antibody
Anti-Mitogen-Activated Protein Kinase 2 antibody
Anti-Map Kinase 2 antibody
Anti-Mapk 2 antibody
Anti-MAPK1 antibody
Anti-ERK2 antibody
Anti-PRKM1 antibody
Anti-PRKM2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance