Anti-MAP3K8 antibody (1-100) (STJ118071)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MAP3K8 (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP3K8 |
| Gene ID | 1326 |
| Uniprot ID | M3K8_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MEYMSTGSDNKEEIDLLIKH LNVSDVIDIMENLYASEEPA VYEPSLMTMCQDSNQNDERS KSLLLSGQEVPWLSSVRYGT VEDLLAFANHISNTAKHFYG |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K8 (NP_005195.2). |
Additional Info
| Tissue Specificity | Expressed in several normal tissues and human tumor-derived cell lines. |
| Post Translational Modifications | Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290.the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB.the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages. |
| Function | Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 8Cancer Osaka Thyroid OncogeneProto-Oncogene C-CotSerine/Threonine-Protein Kinase CotTumor Progression Locus 2Tpl-2 |
| Database Links | Reactome: R-HSA-389357Reactome: R-HSA-5684264 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 antibodyAnti-Cancer Osaka Thyroid Oncogene antibodyAnti-Proto-Oncogene C-Cot antibodyAnti-Serine/Threonine-Protein Kinase Cot antibodyAnti-Tumor Progression Locus 2 antibodyAnti-Tpl-2 antibodyAnti-MAP3K8 antibodyAnti-COT antibodyAnti-ESTF antibody |
Information sourced from Uniprot.org