| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Mitogen-activated protein kinase kinase kinase 11 (640-689) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP3K11 |
| Gene ID: | 4296 |
| Uniprot ID: | M3K11_HUMAN |
| Immunogen Region: | 640-689 |
| Specificity: | MLK3 Polyclonal Antibody detects endogenous levels of MLK3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MLK3. AA range:640-689 |
| Tissue Specificity | Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain. |
| Post Translational Modifications | Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42. |
| Function | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 11 Mixed Lineage Kinase 3 Src-Homology 3 Domain-Containing Proline-Rich Kinase |
| Database Links | Reactome: R-HSA-5673000 Reactome: R-HSA-6802946 Reactome: R-HSA-6802955 Reactome: R-HSA-9013148 Reactome: R-HSA-9013408 Reactome: R-HSA-9013424 Reactome: R-HSA-9649948 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Location Is Cell Cycle Dependent |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 11 antibody Anti-Mixed Lineage Kinase 3 antibody Anti-Src-Homology 3 Domain-Containing Proline-Rich Kinase antibody Anti-MAP3K11 antibody Anti-MLK3 antibody Anti-PTK1 antibody Anti-SPRK antibody |
Information sourced from Uniprot.org