| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP2K4 |
| Gene ID: | 6416 |
| Uniprot ID: | MP2K4_HUMAN |
| Immunogen Region: | 300-399 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1). |
| Immunogen Sequence: | LATGRFPYPKWNSVFDQLTQ VVKGDPPQLSNSEEREFSPS FINFVNLCLTKDESKRPKYK ELLKHPFILMYEERAVEVAC YVCKILDQMPATPSSPMYVD |
| Tissue Specificity | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. |
| Post Translational Modifications | Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks). |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 4 Map Kinase Kinase 4 Mapkk 4 Jnk-Activating Kinase 1 Mapk/Erk Kinase 4 Mek 4 Sapk/Erk Kinase 1 Sek1 Stress-Activated Protein Kinase Kinase 1 Sapk Kinase 1 Sapkk-1 Sapkk1 C-Jun N-Terminal Kinase Kinase 1 Jnkk |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-2871796 Reactome: R-HSA-450321 Reactome: R-HSA-5210891 Reactome: R-HSA-5684264 |
| Cellular Localisation | Cytoplasm Nucleus |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 4 antibody Anti-Map Kinase Kinase 4 antibody Anti-Mapkk 4 antibody Anti-Jnk-Activating Kinase 1 antibody Anti-Mapk/Erk Kinase 4 antibody Anti-Mek 4 antibody Anti-Sapk/Erk Kinase 1 antibody Anti-Sek1 antibody Anti-Stress-Activated Protein Kinase Kinase 1 antibody Anti-Sapk Kinase 1 antibody Anti-Sapkk-1 antibody Anti-Sapkk1 antibody Anti-C-Jun N-Terminal Kinase Kinase 1 antibody Anti-Jnkk antibody Anti-MAP2K4 antibody Anti-JNKK1 antibody Anti-MEK4 antibody Anti-MKK4 antibody Anti-PRKMK4 antibody Anti-SEK1 antibody Anti-SERK1 antibody Anti-SKK1 antibody |
Information sourced from Uniprot.org
