| Host: |
Mouse |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-MAP1LC3B (1-125) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5MM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2ak |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP1LC3B |
| Gene ID: |
81631 |
| Uniprot ID: |
MLP3B_HUMAN |
| Immunogen Region: |
1-125 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3B (NP_073729.1). |
| Immunogen Sequence: |
MPSEKTFKQRRTFEQRVEDV RLIREQHPTKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM SELIKIIRRRLQLNANQAFF LLVNGHSMVSVSTPISEVYE SEKDEDGFLYMVYASQETFG MKLSV |
| Tissue Specificity | Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. |
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation by PKA inhibits conjugation of phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity. |
| Function | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy. |
| Protein Name | Microtubule-Associated Proteins 1a/1b Light Chain 3bAutophagy-Related Protein Lc3 BAutophagy-Related Ubiquitin-Like Modifier Lc3 BMap1 Light Chain 3-Like Protein 2Map1a/Map1b Light Chain 3 BMap1a/Map1b Lc3 BMicrotubule-Associated Protein 1 Light Chain 3 Beta |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685Reactome: R-HSA-8854214Reactome: R-HSA-8934903Reactome: R-HSA-9664873Reactome: R-HSA-9679504Reactome: R-HSA-9694676Reactome: R-HSA-9754560Reactome: R-HSA-9755511 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosome MembraneLipid-AnchorEndomembrane SystemMitochondrion MembraneCytoplasmCytoskeletonLc3-Ii Binds To The Autophagic MembranesLc3-Ii Localizes With The Mitochondrial Inner Membrane During Parkin-Mediated MitophagyLocalizes Also To Discrete Punctae Along The Ciliary Axoneme |
| Alternative Antibody Names | Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3b antibodyAnti-Autophagy-Related Protein Lc3 B antibodyAnti-Autophagy-Related Ubiquitin-Like Modifier Lc3 B antibodyAnti-Map1 Light Chain 3-Like Protein 2 antibodyAnti-Map1a/Map1b Light Chain 3 B antibodyAnti-Map1a/Map1b Lc3 B antibodyAnti-Microtubule-Associated Protein 1 Light Chain 3 Beta antibodyAnti-MAP1LC3B antibodyAnti-MAP1ALC3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
