Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (STJ27585) at 1:500 dilution. 293T cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. C6 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-MAP1LC3A/MAP1LC3B antibody (1-60) (STJ27585)

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STJ27585
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP1LC3B
MAP1LC3A
Gene ID: 81631
84557
Uniprot ID: MLP3B_HUMAN
MLP3A_HUMAN
Immunogen Region: 1-60
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-60 of human LC3A (NP_115903.1).
Immunogen Sequence: MPSDRPFKQRRSFADRCKEV QQIRDQHPSKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM
Tissue Specificity Q9GZQ8 - Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
Q9H492 - Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Alternative Antibody Names Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3b antibody
Anti-Autophagy-Related Protein Lc3 B antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 B antibody
Anti-Map1 Light Chain 3-Like Protein 2 antibody
Anti-Map1a/Map1b Light Chain 3 B antibody
Anti-Map1a/Map1b Lc3 B antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Beta antibody
Anti-MAP1LC3B antibody
Anti-MAP1ALC3 antibody
Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibody
Anti-Autophagy-Related Protein Lc3 A antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibody
Anti-Map1 Light Chain 3-Like Protein 1 antibody
Anti-Map1a/Map1b Light Chain 3 A antibody
Anti-Map1a/Map1b Lc3 A antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibody
Anti-MAP1LC3A antibody

Information sourced from Uniprot.org

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