• Western blot analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) rabbit monoclonal antibody (STJ11101367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human colon carcinoma tissue using Monoamine Oxidase B (MAOB) rabbit monoclonal antibody (STJ11101367) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human kidney tissue using Monoamine Oxidase B (MAOB) rabbit monoclonal antibody (STJ11101367) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human liver tissue using Monoamine Oxidase B (MAOB) rabbit monoclonal antibody (STJ11101367) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human tonsil tissue using Monoamine Oxidase B (MAOB) rabbit monoclonal antibody (STJ11101367) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-MAOB antibody (1-100) [S7MR] (STJ11101367)

SKU:
STJ11101367

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Monoamine Oxidase B (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAOB
Gene ID: 4129
Uniprot ID: AOFB_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Monoamine Oxidase B (MAOB) (P27338).
Immunogen Sequence: MSNKCDVVVVGGGISGMAAA KLLHDSGLNVVVLEARDRVG GRTYTLRNQKVKYVDLGGSY VGPTQNRILRLAKELGLETY KVNEVERLIHHVKGKSYPFR
Function Catalyzes the oxidative deamination of primary and some secondary amines such as neurotransmitters, and exogenous amines including the tertiary amine, neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide and participates in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Preferentially degrades benzylamine and phenylethylamine.
Protein Name Amine Oxidase Flavin-Containing B
Monoamine Oxidase Type B
Mao-B
Database Links Reactome: R-HSA-141333
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Amine Oxidase Flavin-Containing B antibody
Anti-Monoamine Oxidase Type B antibody
Anti-Mao-B antibody
Anti-MAOB antibody

Information sourced from Uniprot.org

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