Anti-MANF antibody (100-150 aa) (STJ13101402)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 100-150 of mouse MANF conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-MANF (100-150 aa) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | MANF |
| Gene ID | 7873 |
| Uniprot ID | MANF_HUMAN |
| Immunogen | A synthetic peptide from aa region 100-150 of mouse MANF conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 100-150 aa |
| Specificity | Specific for MANF. |
Additional Info
| Function | Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange. As a result acts as a repressor of the unfolded protein response (UPR) pathway. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. Essential for embryonic lung development. Required for the correct postnatal temporal and structural development of splenic white pulp. Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling. Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury. Required for endochondral ossification in long bones and the skull during postnatal development. |
| Protein Name | Mesencephalic Astrocyte-Derived Neurotrophic FactorArginine-Rich ProteinProtein Armet |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | SecretedEndoplasmic Reticulum LumenSarcoplasmic Reticulum LumenRetained In The Endoplasmic Reticulum (Er)And Sarcoplasmic Reticulum (Sr) Under Normal ConditionsUp-Regulated And Secreted By The Er/Sr In Response To Er Stress And Hypoxia |
| Alternative Antibody Names | Anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor antibodyAnti-Arginine-Rich Protein antibodyAnti-Protein Armet antibodyAnti-MANF antibodyAnti-ARMET antibodyAnti-ARP antibody |
Information sourced from Uniprot.org