Anti-MAGOH antibody (STJA0007112)

SKU:
STJA0007112

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein mago nashi homolog is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAGOH
Gene ID: 4116
Uniprot ID: MGN_HUMAN
Specificity: This antibody detects endogenous levels of MAGOH at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from human MAGOH
Function Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes).specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S).the function is different from the established EJC assembly.
Protein Name Protein Mago Nashi Homolog
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Reactome: R-HSA-9010553
Reactome: R-HSA-975957
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Detected In Granule-Like Structures In The Dendroplasm
Travels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To Mrna
Colocalizes With The Core Ejc
Alyref/Thoc4
Nxf1 And Uap56 In The Nucleus And Nuclear Speckles
Alternative Antibody Names Anti-Protein Mago Nashi Homolog antibody
Anti-MAGOH antibody
Anti-MAGOHA antibody

Information sourced from Uniprot.org

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