Western blot analysis of various lysates using MAGEA11 Rabbit polyclonal antibody (STJ117483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAGEA11 antibody (1-110) (STJ117483)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ117483
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General Information

Short DescriptionRabbit polyclonal MAGEA11 (1-110) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAGEA11
Gene ID4110
Uniprot IDMAGAB_HUMAN
Immunogen Region1-110
Immunogen SequenceMETQFRRGGLGCSPASIKRK KKREDSGDFGLQVSTMFSED DFQSTERAPYGPQLQWSQDL PRVQVFREQANLEDRSPRRT QRITGGEQVLWGPITQIFPT VRPADLTRVI
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human MAGEA11 (NP_005357.2).

Additional Info

Tissue Specificity Expressed in tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma. Expressed in testis, ovary, prostate, cancerous prostate, breast and adrenal tissue.
Function Acts as androgen receptor coregulator that increases androgen receptor activity by modulating the receptors interdomain interaction. May play a role in embryonal development and tumor transformation or aspects of tumor progression.
Protein Name Melanoma-Associated Antigen 11
Cancer/Testis Antigen 1.11
Ct1.11
Mage-11 Antigen
Database Links
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Melanoma-Associated Antigen 11 antibody
Anti-Cancer/Testis Antigen 1.11 antibody
Anti-Ct1.11 antibody
Anti-Mage-11 Antigen antibody
Anti-MAGEA11 antibody
Anti-MAGE11 antibody

Information sourced from Uniprot.org

Citations

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