| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MAGEA1 (1-309) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAGEA1 |
| Gene ID: |
4100 |
| Uniprot ID: |
MAGA1_HUMAN |
| Immunogen Region: |
1-309 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGE-1/MAGE1A (NP_004979.3). |
| Immunogen Sequence: |
MSLEQRSLHCKPEEALEAQQ EALGLVCVQAATSSSSPLVL GTLEEVPTAGSTDPPQSPQG ASAFPTTINFTRQRQPSEGS SSREEEGPSTSCILESLFRA VITKKVADLVGFLLLKYRAR EPVTKAEMLESVIKNYKHCF PEIFGKASESLQLVFGIDVK EADPTGHSYVLVTCLGLSYD GLLGDNQIMPKTGFLIIVLV MIAMEGGHAPEEEIWEELSV MEVYDGREHSAYGEPRKLL |
| Tissue Specificity | Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas. |
| Function | May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. |
| Protein Name | Melanoma-Associated Antigen 1Antigen Mz2-ECancer/Testis Antigen 1.1Ct1.1Mage-1 Antigen |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Melanoma-Associated Antigen 1 antibodyAnti-Antigen Mz2-E antibodyAnti-Cancer/Testis Antigen 1.1 antibodyAnti-Ct1.1 antibodyAnti-Mage-1 Antigen antibodyAnti-MAGEA1 antibodyAnti-MAGE1 antibodyAnti-MAGE1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TOMM34 knockout (KO) HeLa cells, using [KO Validated] TOMM34 Rabbit polyclonal antibody (STJ11100836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89771/358243/STJ11100836_1__34745.1713122577.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TOMM34 knockout (KO) HeLa cells, using [KO Validated] TOMM34 Rabbit polyclonal antibody (STJ11100836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
