Anti-MADCAM1 antibody [ZN158] (STJA0033382)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenFull length human recombinant protein of human MADCAM1 (NP_570116) produced in HEK293T cell.
STJA0033382
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General Information

Short DescriptionMouse monoclonal anti-MADCAM1 for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC-P
HostMouse
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDZN158
IsotypeIgG1
ConjugationUnconjugated
Concentration0.5-1.0 mg/mL
PurificationAffinity Chromatography
Dilution RangeIHC 1:50-1:100
FormulationPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolMADCAM1
Gene ID8174
Uniprot IDMADCA_HUMAN
ImmunogenFull length human recombinant protein of human MADCAM1 (NP_570116) produced in HEK293T cell.

Additional Info

Tissue Specificity Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Post Translational Modifications The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
Function Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Protein Name Mucosal Addressin Cell Adhesion Molecule 1
Madcam-1
Hmadcam-1
Database Links Reactome: R-HSA-198933 Q13477-1
Reactome: R-HSA-216083 Q13477-1
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Mucosal Addressin Cell Adhesion Molecule 1 antibody
Anti-Madcam-1 antibody
Anti-Hmadcam-1 antibody
Anti-MADCAM1 antibody

Information sourced from Uniprot.org

Citations

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