Immunohistochemistry analysis of MADCAM1 in paraffin-embedded human appendix using MADCAM1 Rabbit polyclonal antibody (STJ24442) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MADCAM1 antibody (100-200) (STJ24442)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ24442
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General Information

Short DescriptionRabbit polyclonal MADCAM1 (100-200) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:200-1:1000
IHC-P:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMADCAM1
Gene ID8174
Uniprot IDMADCA_HUMAN
Immunogen Region100-200
Immunogen SequenceGRTFQHTVQLLVYAFPDQLT VSPAALVPGDPEVACTAHKV TPVDPNALSFSLLVGGQELE GAQALGPEVQEEEEEPQGDE DVLFRVTERWRLPPLGTPVP P
SpecificityA synthetic peptide corresponding to a sequence within amino acids 100-200 of human MADCAM1 (NP_570116.2).

Additional Info

Tissue Specificity Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Post Translational Modifications The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
Function Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Protein Name Mucosal Addressin Cell Adhesion Molecule 1
Madcam-1
Hmadcam-1
Database Links Reactome: R-HSA-198933 Q13477-1
Reactome: R-HSA-216083 Q13477-1
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Mucosal Addressin Cell Adhesion Molecule 1 antibody
Anti-Madcam-1 antibody
Anti-Hmadcam-1 antibody
Anti-MADCAM1 antibody

Information sourced from Uniprot.org

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