• Immunohistochemistry analysis of paraffin-embedded human colon using MAD2L1BP Rabbit monoclonal antibody (STJ11103050) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MAD2L1BP Rabbit monoclonal antibody (STJ11103050) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAD2L1BP antibody (175-274) [S0MR] (STJ11103050)

SKU:
STJ11103050

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MAD2L1BP (175-274) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAD2L1BP
Gene ID: 9587
Uniprot ID: MD2BP_HUMAN
Immunogen Region: 175-274
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 175-274 of human MAD2L1BP (Q15013).
Immunogen Sequence: YSVDQSLSTAACLRRLFRAI FMADAFSELQAPPLMGTVVM AQGHRNCGEDWFRPKLNYRV PSRGHKLTVTLSCGRPSIRT TAWEDYIWFQAPVTFKGFRE
Function May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
Protein Name Mad2l1-Binding Protein
Caught By Mad2 Protein
P31(Comet
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
During Early Mitosis
Unevenly Distributed Throughout The Nucleoplasm
From Metaphase To Anaphase
Concentrated On The Spindle
Alternative Antibody Names Anti-Mad2l1-Binding Protein antibody
Anti-Caught By Mad2 Protein antibody
Anti-P31(Comet antibody
Anti-MAD2L1BP antibody
Anti-CMT2 antibody
Anti-KIAA0110 antibody

Information sourced from Uniprot.org

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