Anti-LXR beta/NR1H2 antibody (N-Term) (STJ70603)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-LXR beta/NR1H2 (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/IF/FC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.IF: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ยตg/ml. FC:Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | NR1H2 |
| Gene ID | 7376 |
| Uniprot ID | NR1H2_HUMAN |
| Accession Number | NP_009052.3 |
| Immunogen Region | N-Term |
| Immunogen Sequence | SSPTTSSLDTPLPGC |
| Specificity | Based on the peptide used, this antibody is expected to be specific for LXR beta and not to cross-react with LXR alpha. |
Additional Info
| Post Translational Modifications | Sumoylated by SUMO2 at Lys-409 and Lys-447 during the hepatic acute phase response, leading to promote interaction with GPS2 and prevent N-Cor corepressor complex dissociation. |
| Function | Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. |
| Protein Name | Oxysterols Receptor Lxr-BetaLiver X Receptor BetaNuclear Receptor NerNuclear Receptor Subfamily 1 Group H Member 2Ubiquitously-Expressed Nuclear Receptor |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-383280Reactome: R-HSA-4090294Reactome: R-HSA-8866427Reactome: R-HSA-9029558Reactome: R-HSA-9029569Reactome: R-HSA-9031525Reactome: R-HSA-9031528Reactome: R-HSA-9623433 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Oxysterols Receptor Lxr-Beta antibodyAnti-Liver X Receptor Beta antibodyAnti-Nuclear Receptor Ner antibodyAnti-Nuclear Receptor Subfamily 1 Group H Member 2 antibodyAnti-Ubiquitously-Expressed Nuclear Receptor antibodyAnti-NR1H2 antibodyAnti-LXRB antibodyAnti-NER antibodyAnti-UNR antibody |
Information sourced from Uniprot.org