Anti-LRWD1 antibody (250-350) (STJ11104322)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Leucine-Rich Repeat And Wd Repeat-Containing Protein 1 (250-350) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LRWD1 |
| Gene ID | 222229 |
| Uniprot ID | LRWD1_HUMAN |
| Immunogen Region | 250-350 |
| Immunogen Sequence | ASPSAQVEGSPVAGSDGSQP AVKLEPLHFLQCHSKNNSPQ DLETQLWACAFEPAWEEGAT SQTVATCGGEAVCVIDCQTG IVLHKYKAPGEEFFSVAWTA L |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Lrwd1 (NP_690852.1). |
Additional Info
| Tissue Specificity | Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS). |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination leading to proteasomal degradation. Ubiquitination occurs within the WD repeats at the end of the G1 phase. Ubiquitination may be catalyzed by the CUL4-DDB1 E3 ubiquitin-protein ligase complex and other E3 ligases. |
| Function | Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability. |
| Protein Name | Leucine-Rich Repeat And Wd Repeat-Containing Protein 1Centromere Protein 33Cenp-33Origin Recognition Complex-Associated ProteinOrc-Associated ProteinOrca |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCentromereTelomereCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeKinetochoreLocalizes To Heterochromatin During G1 PhaseRestricted To Centromeres Or Telomeres As Cells Progress Though S PhaseWhen Cells Enter MitosisRelocalizes To CentromeresRecruitment To Pericentric Heterochromatin Largely Depends On The Presence Of H3k9me3 |
| Alternative Antibody Names | Anti-Leucine-Rich Repeat And Wd Repeat-Containing Protein 1 antibodyAnti-Centromere Protein 33 antibodyAnti-Cenp-33 antibodyAnti-Origin Recognition Complex-Associated Protein antibodyAnti-Orc-Associated Protein antibodyAnti-Orca antibodyAnti-LRWD1 antibodyAnti-CENP-33 antibodyAnti-ORCA antibody |
Information sourced from Uniprot.org