• Western blot analysis of lysates from Raji cells using LRRK1 Rabbit polyclonal antibody (STJ11104464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LRRK1 antibody (10-90) (STJ11104464)

SKU:
STJ11104464

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRRK1
Gene ID: 79705
Uniprot ID: LRRK1_HUMAN
Immunogen Region: 10-90
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 10-90 of human LRRK1 (NP_078928.3).
Immunogen Sequence: SMYWCVGPEESAVCPERAME TLNGAGDTGGKPSTRGGDPA ARSRRTEGIRAAYRRGDRGG ARDLLEEACDQCASQLEKGQ L
Post Translational Modifications Autophosphorylated. Autophosphorylation is inhibited in its dimeric state. Phosphorylated by protein kinase C isozymes PRKCA, PRKCB, PRKCG, PRKCE, PRKCZ and PRKCT at Thr-1061, Ser-1064, Ser-1074 and Thr-1075. Phosphorylation at these Ser-1064, Ser-1074 and Thr-1075 activates the kinase activity of LRRK1 to phosphorylate RAB7A.
Function Serine/threonine-protein kinase which phosphorylates RAB proteins involved in intracellular trafficking. Phosphorylates RAB7A.this activity is dependent on protein kinase C (PKC) activation. Plays a role in the negative regulation of bone mass, acting through the maturation of osteoclasts.
Protein Name Leucine-Rich Repeat Serine/Threonine-Protein Kinase 1
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Leucine-Rich Repeat Serine/Threonine-Protein Kinase 1 antibody
Anti-LRRK1 antibody
Anti-KIAA1790 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance