Anti-LMNB1 antibody (397-588) (STJ24413)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Lamin B1 (397-588) antibody for use in WB, IHC-P, IF, ICC, IP, ELISA and ChIP in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LMNB1 |
| Gene ID | 4001 |
| Uniprot ID | LMNB1_HUMAN |
| Immunogen Region | 397-588 |
| Immunogen Sequence | RVTVSRASSSRSVRTTRGKR KRVDVEESEASSSVSISHSA SATGNVCIEEIDVDGKFIRL KNTSEQDQPMGGWEMIRKIG DTSVSYKYTSRYVLKAGQTV TIWAANAGVTASPPTDLIWK NQNSWGTGEDVKVILKNSQG EEVAQRSTVFKTTIPEEEEE EEEAAGVVVEEELFHQQGTP RASNRSCAIM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1). |
Additional Info
| Post Translational Modifications | B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Phosphorylation plays a key role in lamin organization, subcellular localization and nuclear envelope disintegration. Phosphorylation by CDK1 at Ser-23 and Ser-393 at the onset of mitosis drives lamin disassembly and nuclear envelope breakdown. |
| Function | Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. |
| Protein Name | Lamin-B1 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-2559584Reactome: R-HSA-2980766Reactome: R-HSA-2995383Reactome: R-HSA-352238Reactome: R-HSA-4419969Reactome: R-HSA-8862803Reactome: R-HSA-8950505Reactome: R-HSA-9013405Reactome: R-HSA-9035034 |
| Cellular Localisation | Nucleus Lamina |
| Alternative Antibody Names | Anti-Lamin-B1 antibodyAnti-LMNB1 antibodyAnti-LMN2 antibodyAnti-LMNB antibody |
Information sourced from Uniprot.org