• Immunohistochemistry analysis of paraffin-embedded human placenta using LMAN1 Rabbit monoclonal antibody (STJ11102202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using LMAN1 Rabbit monoclonal antibody (STJ11102202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using LMAN1 Rabbit monoclonal antibody (STJ11102202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using LMAN1 Rabbit monoclonal antibody (STJ11102202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LMAN1 antibody (1-100) [S2MR] (STJ11102202)

SKU:
STJ11102202

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LMAN1 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMAN1
Gene ID: 3998
Uniprot ID: LMAN1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LMAN1 (P49257).
Immunogen Sequence: MAGSRQRGLRARVRPLFCAL LLSLGRFVRGDGVGGDPAVA LPHRRFEYKYSFKGPHLVQS DGTVPFWAHAGNAIPSSDQI RVAPSLKSQRGSVWTKTKAA
Tissue Specificity Ubiquitous.
Post Translational Modifications The N-terminal may be partly blocked.
Function Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
Protein Name Protein Ergic-53
Er-Golgi Intermediate Compartment 53 Kda Protein
Gp58
Intracellular Mannose-Specific Lectin Mr60
Lectin Mannose-Binding 1
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013408
Reactome: R-HSA-9013423
Reactome: R-HSA-948021
Cellular Localisation Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus Membrane
Single-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Protein Ergic-53 antibody
Anti-Er-Golgi Intermediate Compartment 53 Kda Protein antibody
Anti-Gp58 antibody
Anti-Intracellular Mannose-Specific Lectin Mr60 antibody
Anti-Lectin Mannose-Binding 1 antibody
Anti-LMAN1 antibody
Anti-ERGIC53 antibody
Anti-F5F8D antibody

Information sourced from Uniprot.org

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