| Host: |
Goat |
| Applications: |
Pep-ELISA/IF/FC |
| Reactivity: |
Human/Mouse/Dog |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Lipin 1 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.IF: Strong expression of the protein seen in the cytoplasm and plasma membranes of U2OS cells. Recommended concentration: 10µg/ml. FC:Flow cytometric analysis of U2OS cells. Recommended |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
LPIN1 |
| Gene ID: |
23175 |
| Uniprot ID: |
LPIN1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_663731.1 |
| Immunogen Sequence: |
NKTDSPSRKRDK |
| Post Translational Modifications | Phosphorylated at multiple sites by mTOR in response to insulin, leading to its inactivation. Phosphorylation does not affect the catalytic activity but regulates the localization. Phosphorylation is decreased by epinephrine. Dephosphorylated by the CTDNEP1-CNEP1R1 complex. Dephosphorylation following mTOR inhibition promotes its activity. Acetylation at Lys-425 and Lys-595 by KAT5 in response to fatty acids promotes translocation to the endoplasmic reticulum and synthesis of diacylglycerol. Sumoylated. |
| Function | Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. Is involved in adipocyte differentiation. Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. |
| Protein Name | Phosphatidate Phosphatase Lpin1Lipin-1 |
| Database Links | Reactome: R-HSA-1483191Reactome: R-HSA-1483213Reactome: R-HSA-4419969Reactome: R-HSA-75109 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic Reticulum MembraneNucleus MembraneTranslocates From The Cytosol To The Endoplasmic Reticulum Following Acetylation By Kat5 |
| Alternative Antibody Names | Anti-Phosphatidate Phosphatase Lpin1 antibodyAnti-Lipin-1 antibodyAnti-LPIN1 antibodyAnti-KIAA0188 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
