| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LIN28 (100-209) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LIN28A |
| Gene ID: |
79727 |
| Uniprot ID: |
LN28A_HUMAN |
| Immunogen Region: |
100-209 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 100-209 of human LIN28A (NP_078950.1). |
| Immunogen Sequence: |
SAKGLESIRVTGPGGVFCIG SERRPKGKSMQKRRSKGDRC YNCGGLDHHAKECKLPPQPK KCHFCQSISHMVASCPLKAQ QGPSAQGKPTYFREEEEEIH SPTLLPEAQN |
| Tissue Specificity | Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors. |
| Function | RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells. Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue. |
| Protein Name | Protein Lin-28 Homolog ALin-28aZinc Finger Cchc Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-452723 |
| Cellular Localisation | CytoplasmRough Endoplasmic ReticulumP-BodyStress GranuleNucleusNucleolusPredominantly CytoplasmicIn The CytoplasmLocalizes To Peri-Endoplasmic Reticulum Regions And Detected In The Microsomal Fraction Derived From Rough Endoplasmic Reticulum (Rer) Following Subcellular FractionationMay Be Bound To The Cytosolic Surface Of Rer On Which Er-Associated Mrnas Are TranslatedShuttle From The Nucleus To The Cytoplasm Requires Rna-BindingNucleolar Localization Is Observed In 10-15% Of The Nuclei In Differentiated Myotubes |
| Alternative Antibody Names | Anti-Protein Lin-28 Homolog A antibodyAnti-Lin-28a antibodyAnti-Zinc Finger Cchc Domain-Containing Protein 1 antibodyAnti-LIN28A antibodyAnti-CSDD1 antibodyAnti-LIN28 antibodyAnti-ZCCHC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
