• Anti-LIMS1 antibody [5G7] (STJ98322)
  • Immunofluorescence analysis of HepG2 cells using Pinch-1 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of Hela cells using Pinch-1 monoclonal antibody (blue) and negative control (red).
  • Western blot analysis using Pinch-1 monoclonal antibody against A549 (1) , Jurkat (2) , and HeLa (3) cell lysate.

Anti-LIMS1 antibody [5G7] (STJ98322)

SKU:
STJ98322

Current Stock:
Host: Mouse
Applications: WB/IF/FC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-LIM and senescent cell antigen-like-containing domain protein 1 is suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 5G7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIMS1
Gene ID: 3987
Uniprot ID: LIMS1_HUMAN
Specificity: Pinch-1 Monoclonal Antibody detects endogenous levels of Pinch-1 protein.
Immunogen: Purified recombinant fragment of human Pinch-1 expressed in E. Coli.
Function Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.
Protein Name Lim And Senescent Cell Antigen-Like-Containing Domain Protein 1
Particularly Interesting New Cys-His Protein 1
Pinch-1
Renal Carcinoma Antigen Ny-Ren-48
Database Links Reactome: R-HSA-446353
Reactome: R-HSA-446388
Cellular Localisation Cell Junction
Focal Adhesion
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Lim And Senescent Cell Antigen-Like-Containing Domain Protein 1 antibody
Anti-Particularly Interesting New Cys-His Protein 1 antibody
Anti-Pinch-1 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-48 antibody
Anti-LIMS1 antibody
Anti-PINCH antibody
Anti-PINCH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance