• Immunofluorescence analysis of NIH/3T3 cells using LIMS1 Rabbit polyclonal antibody (STJ115853) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse heart, using LIMS1 antibody (STJ115853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-LIMS1 antibody (1-325) (STJ115853)

SKU:
STJ115853

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIMS1 (1-325) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIMS1
Gene ID: 3987
Uniprot ID: LIMS1_HUMAN
Immunogen Region: 1-325
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human LIMS1 (NP_001180417.1).
Immunogen Sequence: MANALASATCERCKGGFAPA EKIVNSNGELYHEQCFVCAQ CFQQFPEGLFYEFEGRKYCE HDFQMLFAPCCHQCGEFIIG RVIKAMNNSWHPECFRCDLC QEVLADIGFVKNAGRHLCRP CHNREKARGLGKYICQKCHA IIDEQPLIFKNDPYHPDHFN CANCGKELTADARELKGELY CLPCHDKMGVPICGACRRPI EGRVVNAMGKQWHVEHFVCA KCEKPFLGHRHYERKGLAY
Tissue Specificity Expressed in most tissues except in the brain.
Function Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.
Protein Name Lim And Senescent Cell Antigen-Like-Containing Domain Protein 1
Particularly Interesting New Cys-His Protein 1
Pinch-1
Renal Carcinoma Antigen Ny-Ren-48
Database Links Reactome: R-HSA-446353
Reactome: R-HSA-446388
Cellular Localisation Cell Junction
Focal Adhesion
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Lim And Senescent Cell Antigen-Like-Containing Domain Protein 1 antibody
Anti-Particularly Interesting New Cys-His Protein 1 antibody
Anti-Pinch-1 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-48 antibody
Anti-LIMS1 antibody
Anti-PINCH antibody
Anti-PINCH1 antibody

Information sourced from Uniprot.org

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