| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LIG4 (1-210) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LIG4 |
| Gene ID: |
3981 |
| Uniprot ID: |
DNLI4_HUMAN |
| Immunogen Region: |
1-210 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DNA Ligase IV (NP_002303.2). |
| Immunogen Sequence: |
MAASQTSQTVASHVPFADLC STLERIQKSKGRAEKIRHFR EFLDSWRKFHDALHKNHKDV TDSFYPAMRLILPQLERERM AYGIKETMLAKLYIELLNLP RDGKDALKLLNYRTPTGTHG DAGDFAMIAYFVLKPRCLQK GSLTIQQVNDLLDSIASNNS AKRKDLIKKSLLQLITQSSA LEQKWLIRMIIKDLKLGVSQ QTIFSVFHND |
| Tissue Specificity | Testis, thymus, prostate and heart. |
| Function | DNA ligase involved in DNA non-homologous end joining (NHEJ).required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4.the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. LIG4 regulates nuclear localization of XRCC4. |
| Protein Name | Dna Ligase 4Dna Ligase IvPolydeoxyribonucleotide Synthase Atp 4 |
| Database Links | Reactome: R-HSA-164843Reactome: R-HSA-5693571 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Ligase 4 antibodyAnti-Dna Ligase Iv antibodyAnti-Polydeoxyribonucleotide Synthase Atp 4 antibodyAnti-LIG4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit polyclonal antibody (STJ11100023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89095/357554/STJ11100023_1__39011.1713121676.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit polyclonal antibody (STJ11100023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
