• Confocal imaging of paraffin-embedded Mouse colon tissue using Galectin 4/LGALS4 Rabbit monoclonal antibody (STJ11101927, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Human colon cancer tissue using Galectin 4/LGALS4 Rabbit monoclonal antibody (STJ11101927, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat colon tissue using Galectin 4/LGALS4 Rabbit monoclonal antibody (STJ11101927, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Galectin 4/LGALS4 in paraffin-embedded mouse colon using Galectin 4/LGALS4 Rabbit monoclonal antibody (STJ11101927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Galectin 4/LGALS4 in paraffin-embedded human appendix using Galectin 4/LGALS4 Rabbit monoclonal antibody (STJ11101927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Galectin 4/LGALS4 Rabbit monoclonal antibody (STJ11101927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-LGALS4 antibody (1-100) [S7MR] (STJ11101927)

SKU:
STJ11101927

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:4000
IHC-P:1:100-1:1000
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LGALS4
Gene ID: 3960
Uniprot ID: LEG4_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Galectin 4/LGALS4 (P56470).
Immunogen Sequence: MAYVPAPGYQPTYNPTLPYY QPIPGGLNVGMSVYIQGVAS EHMKRFFVNFVVGQDPGSDV AFHFNPRFDGWDKVVFNTLQ GGKWGSEERKRSMPFKKGAA
Function Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Protein Name Galectin-4
Gal-4
Antigen Ny-Co-27
L-36 Lactose-Binding Protein
L36lbp
Lactose-Binding Lectin 4
Alternative Antibody Names Anti-Galectin-4 antibody
Anti-Gal-4 antibody
Anti-Antigen Ny-Co-27 antibody
Anti-L-36 Lactose-Binding Protein antibody
Anti-L36lbp antibody
Anti-Lactose-Binding Lectin 4 antibody
Anti-LGALS4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance