| Host: |
Mouse |
| Applications: |
ELISA/FC/WB/IF/IHC |
| Reactivity: |
Human/Guinea Pig |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Lewis b is suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
2-25LE |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
ELISA (solid phase: not known; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0.5-1.0 µg/million cells in 0.1 ml). Immunoblotting (1-2 µg/ml). Immunofluorescence (0.5-1 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of for |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
FUT3 |
| Gene ID: |
2525 |
| Uniprot ID: |
FUT3_HUMAN |
| Immunogen: |
A BALB/c mouse was immunized with ALea-b+ ovarian cyst mucin. Fusion partner: SP-2/0. |
| Tissue Specificity | Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver. |
| Post Translational Modifications | Glycosylated. |
| Function | Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to both the subterminal N-acetyl glucosamine (GlcNAc) of type 1 chain (beta-D-Gal-(1->3)-beta-D-GlcNAc) glycolipids and oligosaccharides via an alpha(1,4) linkage, and the subterminal glucose (Glc) or GlcNAc of type 2 chain (beta-D-Gal-(1->4)-beta-D-GlcNAc) oligosaccharides via an alpha(1,3) linkage, independently of the presence of terminal alpha-L-fucosyl-(1,2) moieties on the terminal galactose of these acceptors and participates in the blood groups Lewis determination and expression of Lewis a (Le(a)), lewis b (Le(b)), Lewis x/SSEA-1 (Le(x)) and lewis y (Le(y)) antigens. Also catalyzes the transfer of L-fucose to subterminal GlcNAc of sialyl- and disialyl-lactotetraosylceramide to produce sialyl Lewis a (sLe(a)) and disialyl Lewis a via an alpha(1,4) linkage and therefore may regulate cell surface sialyl Lewis a expression and consequently regulates adhesive properties to E-selectin, cell proliferation and migration. Catalyzes the transfer of an L-fucose to 3'-sialyl-N-acetyllactosamine by an alpha(1,3) linkage, which allows the formation of sialyl-Lewis x structure and therefore may regulate the sialyl-Lewis x surface antigen expression and consequently adhesive properties to E-selectin. Prefers type 1 chain over type 2 acceptors. Type 1 tetrasaccharide is a better acceptor than type 1 disaccharide suggesting that a beta anomeric configuration of GlcNAc in the substrate is preferred. Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. |
| Protein Name | 3-Galactosyl-N-Acetylglucosaminide 4-Alpha-L-Fucosyltransferase Fut34-Galactosyl-N-Acetylglucosaminide 3-Alpha-L-FucosyltransferaseAlpha-3-Fucosyltransferase Fut3Blood Group Lewis Alpha-4-FucosyltransferaseLewis FtFucosyltransferase 3Fucosyltransferase IiiFuct-Iii |
| Database Links | Reactome: R-HSA-9037629Reactome: R-HSA-975578 |
| Cellular Localisation | Golgi ApparatusGolgi Stack MembraneSingle-Pass Type Ii Membrane ProteinMembrane-Bound Form In Trans Cisternae Of Golgi |
| Alternative Antibody Names | Anti-3-Galactosyl-N-Acetylglucosaminide 4-Alpha-L-Fucosyltransferase Fut3 antibodyAnti-4-Galactosyl-N-Acetylglucosaminide 3-Alpha-L-Fucosyltransferase antibodyAnti-Alpha-3-Fucosyltransferase Fut3 antibodyAnti-Blood Group Lewis Alpha-4-Fucosyltransferase antibodyAnti-Lewis Ft antibodyAnti-Fucosyltransferase 3 antibodyAnti-Fucosyltransferase Iii antibodyAnti-Fuct-Iii antibodyAnti-FUT3 antibodyAnti-FT3B antibodyAnti-LE antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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