• Anti-Lewis b antibody [2-25LE] (STJ16100268)

Anti-Lewis b antibody [2-25LE] (STJ16100268)

SKU:
STJ16100268

£264.50 - £1,251.50
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Host: Mouse
Applications: ELISA/FC/WB/IF/IHC
Reactivity: Human/Guinea Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Lewis b is suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 2-25LE
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: ELISA (solid phase: not known; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0.5-1.0 µg/million cells in 0.1 ml). Immunoblotting (1-2 µg/ml). Immunofluorescence (0.5-1 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of for
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: FUT3
Gene ID: 2525
Uniprot ID: FUT3_HUMAN
Immunogen: A BALB/c mouse was immunized with ALea-b+ ovarian cyst mucin. Fusion partner: SP-2/0.
Tissue Specificity Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
Post Translational Modifications Glycosylated.
Function Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to both the subterminal N-acetyl glucosamine (GlcNAc) of type 1 chain (beta-D-Gal-(1->3)-beta-D-GlcNAc) glycolipids and oligosaccharides via an alpha(1,4) linkage, and the subterminal glucose (Glc) or GlcNAc of type 2 chain (beta-D-Gal-(1->4)-beta-D-GlcNAc) oligosaccharides via an alpha(1,3) linkage, independently of the presence of terminal alpha-L-fucosyl-(1,2) moieties on the terminal galactose of these acceptors and participates in the blood groups Lewis determination and expression of Lewis a (Le(a)), lewis b (Le(b)), Lewis x/SSEA-1 (Le(x)) and lewis y (Le(y)) antigens. Also catalyzes the transfer of L-fucose to subterminal GlcNAc of sialyl- and disialyl-lactotetraosylceramide to produce sialyl Lewis a (sLe(a)) and disialyl Lewis a via an alpha(1,4) linkage and therefore may regulate cell surface sialyl Lewis a expression and consequently regulates adhesive properties to E-selectin, cell proliferation and migration. Catalyzes the transfer of an L-fucose to 3'-sialyl-N-acetyllactosamine by an alpha(1,3) linkage, which allows the formation of sialyl-Lewis x structure and therefore may regulate the sialyl-Lewis x surface antigen expression and consequently adhesive properties to E-selectin. Prefers type 1 chain over type 2 acceptors. Type 1 tetrasaccharide is a better acceptor than type 1 disaccharide suggesting that a beta anomeric configuration of GlcNAc in the substrate is preferred. Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme.
Protein Name 3-Galactosyl-N-Acetylglucosaminide 4-Alpha-L-Fucosyltransferase Fut3
4-Galactosyl-N-Acetylglucosaminide 3-Alpha-L-Fucosyltransferase
Alpha-3-Fucosyltransferase Fut3
Blood Group Lewis Alpha-4-Fucosyltransferase
Lewis Ft
Fucosyltransferase 3
Fucosyltransferase Iii
Fuct-Iii
Database Links Reactome: R-HSA-9037629
Reactome: R-HSA-975578
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Membrane-Bound Form In Trans Cisternae Of Golgi
Alternative Antibody Names Anti-3-Galactosyl-N-Acetylglucosaminide 4-Alpha-L-Fucosyltransferase Fut3 antibody
Anti-4-Galactosyl-N-Acetylglucosaminide 3-Alpha-L-Fucosyltransferase antibody
Anti-Alpha-3-Fucosyltransferase Fut3 antibody
Anti-Blood Group Lewis Alpha-4-Fucosyltransferase antibody
Anti-Lewis Ft antibody
Anti-Fucosyltransferase 3 antibody
Anti-Fucosyltransferase Iii antibody
Anti-Fuct-Iii antibody
Anti-FUT3 antibody
Anti-FT3B antibody
Anti-LE antibody

Information sourced from Uniprot.org

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