| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LEF1 |
| Gene ID: | 51176 |
| Uniprot ID: | LEF1_HUMAN |
| Immunogen Region: | 20-132 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-132 of human LEF1 (NP_057353.1). |
| Immunogen Sequence: | TDEMIPFKDEGDPQKEKIFA EISHPEEEGDLADIKSSLVN ESEIIPASNGHEVARQAQTS QEPYHDKAREHPDDGKHPDG GLYNKGPSYSSYSGYIMMPN MNNDPYMSNGSLS |
| Tissue Specificity | Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. |
| Post Translational Modifications | Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway. |
| Function | Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling. May play a role in hair cell differentiation and follicle morphogenesis. Isoform 1: Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. Isoform 3: Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling. Isoform 5: Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. |
| Protein Name | Lymphoid Enhancer-Binding Factor 1 Lef-1 T Cell-Specific Transcription Factor 1-Alpha Tcf1-Alpha |
| Database Links | Reactome: R-HSA-201722 Reactome: R-HSA-3769402 Reactome: R-HSA-4086398 Reactome: R-HSA-4411364 Reactome: R-HSA-4641265 Reactome: R-HSA-8853884 Reactome: R-HSA-8951430 Reactome: R-HSA-9616222 Reactome: R-HSA-9733709 Reactome: R-HSA-9754189 Reactome: R-HSA-9793380 Reactome: R-HSA-9796292 Reactome: R-HSA-9824272 Reactome: R-HSA-9824585 Reactome: R-HSA-9825892 Reactome: R-HSA-9856649 |
| Cellular Localisation | Nucleus Found In Nuclear Bodies Upon Piasg Binding |
| Alternative Antibody Names | Anti-Lymphoid Enhancer-Binding Factor 1 antibody Anti-Lef-1 antibody Anti-T Cell-Specific Transcription Factor 1-Alpha antibody Anti-Tcf1-Alpha antibody Anti-LEF1 antibody |
Information sourced from Uniprot.org