• Western blot analysis of extracts from normal (control) and LDHB knockout (KO) 293T cells, using LDHB antibody (STJ11100069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts from wild type (WT) and LDHB knockout (KO) 293T cells, using LDHB rabbit polyclonal antibody antibody (STJ11100069) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-LDHB antibody (2-334) (STJ11100069)

SKU:
STJ11100069

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHB
Gene ID: 3945
Uniprot ID: LDHB_HUMAN
Immunogen Region: 2-334
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 2-334 of human LDHB (NP_002291.1).
Immunogen Sequence: ATLKEKLIAPVAEEEATVPN NKITVVGVGQVGMACAISIL GKSLADELALVDVLEDKLKG EMMDLQHGSLFLQTPKIVAD KDYSVTANSKIVVVTAGVRQ QEGESRLNLVQRNVNVFKFI IPQIVKYSPDCIIIVVSNPV DILTYVTWKLSGLPKHRVIG SGCNLDSARFRYLMAEKLGI HPSSCHGWILGEHGDSSVAV WSGVNVAGVSLQELNPEMGT DNDSENWKEVHKMVVESAY
Tissue Specificity Predominantly expressed in aerobic tissues such as cardiac muscle.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase B Chain
Ldh-B
Ldh Heart Subunit
Ldh-H
Renal Carcinoma Antigen Ny-Ren-46
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Mitochondrion Inner Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-L-Lactate Dehydrogenase B Chain antibody
Anti-Ldh-B antibody
Anti-Ldh Heart Subunit antibody
Anti-Ldh-H antibody
Anti-Renal Carcinoma Antigen Ny-Ren-46 antibody
Anti-LDHB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance