• Immunohistochemistry analysis of LDHB in paraffin-embedded Human colon tissue using LDHB Rabbit polyclonal antibody (STJ29762) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHB in paraffin-embedded Mouse heart tissue using LDHB Rabbit polyclonal antibody (STJ29762) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHB in paraffin-embedded Rat heart tissue using LDHB Rabbit polyclonal antibody (STJ29762) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using LDHB Rabbit polyclonal antibody (STJ29762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LDHB antibody (1-334) (STJ29762)

SKU:
STJ29762

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LDHB (1-334) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHB
Gene ID: 3945
Uniprot ID: LDHB_HUMAN
Immunogen Region: 1-334
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human LDHB (NP_002291.1).
Immunogen Sequence: MATLKEKLIAPVAEEEATVP NNKITVVGVGQVGMACAISI LGKSLADELALVDVLEDKLK GEMMDLQHGSLFLQTPKIVA DKDYSVTANSKIVVVTAGVR QQEGESRLNLVQRNVNVFKF IIPQIVKYSPDCIIIVVSNP VDILTYVTWKLSGLPKHRVI GSGCNLDSARFRYLMAEKLG IHPSSCHGWILGEHGDSSVA VWSGVNVAGVSLQELNPEMG TDNDSENWKEVHKMVVESA
Tissue Specificity Predominantly expressed in aerobic tissues such as cardiac muscle.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase B Chain
Ldh-B
Ldh Heart Subunit
Ldh-H
Renal Carcinoma Antigen Ny-Ren-46
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Mitochondrion Inner Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-L-Lactate Dehydrogenase B Chain antibody
Anti-Ldh-B antibody
Anti-Ldh Heart Subunit antibody
Anti-Ldh-H antibody
Anti-Renal Carcinoma Antigen Ny-Ren-46 antibody
Anti-LDHB antibody

Information sourced from Uniprot.org

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