• Immunofluorescence analysis of MCF7 cells using LDHA Rabbit polyclonal antibody (STJ24386) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using LDHA Rabbit polyclonal antibody (STJ24386) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded mouse brain tissue using LDHA Rabbit polyclonal antibody (STJ24386) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded human liver tissue using LDHA Rabbit polyclonal antibody (STJ24386) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded human colon tissue using LDHA Rabbit polyclonal antibody (STJ24386) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded rat large intestine tissue using LDHA Rabbit polyclonal antibody (STJ24386) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded mouse kidney tissue using LDHA Rabbit polyclonal antibody (STJ24386) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded rat kidney tissue using LDHA Rabbit polyclonal antibody (STJ24386) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded rat liver tissue using LDHA Rabbit polyclonal antibody (STJ24386) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from HeLa cells using LDHA Rabbit polyclonal antibody (STJ24386) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using LDHA Rabbit polyclonal antibody (STJ24386) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from Rat liver using LDHA Rabbit polyclonal antibody (STJ24386) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LDHA antibody (1-332) (STJ24386)

SKU:
STJ24386

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LDHA (1-332) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHA
Gene ID: 3939
Uniprot ID: LDHA_HUMAN
Immunogen Region: 1-332
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1).
Immunogen Sequence: MATLKDQLIYNLLKEEQTPQ NKITVVGVGAVGMACAISIL MKDLADELALVDVIEDKLKG EMMDLQHGSLFLRTPKIVSG KDYNVTANSKLVIITAGARQ QEGESRLNLVQRNVNIFKFI IPNVVKYSPNCKLLIVSNPV DILTYVAWKISGFPKNRVIG SGCNLDSARFRYLMGERLGV HPLSCHGWVLGEHGDSSVPV WSGMNVAGVSLKTLHPDLGT DKDKEQWKEVHKQVVESAY
Tissue Specificity Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Post Translational Modifications ISGylated.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase A Chain
Ldh-A
Cell Proliferation-Inducing Gene 19 Protein
Ldh Muscle Subunit
Ldh-M
Renal Carcinoma Antigen Ny-Ren-59
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-L-Lactate Dehydrogenase A Chain antibody
Anti-Ldh-A antibody
Anti-Cell Proliferation-Inducing Gene 19 Protein antibody
Anti-Ldh Muscle Subunit antibody
Anti-Ldh-M antibody
Anti-Renal Carcinoma Antigen Ny-Ren-59 antibody
Anti-LDHA antibody
Anti-PIG19 antibody

Information sourced from Uniprot.org

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