• Immunofluorescence analysis of mouse heart cells using LDB3 antibody (STJ29598) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat heart cells using LDB3 antibody (STJ29598) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human placenta cells using LDB3 antibody (STJ29598) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using LDB3 Rabbit polyclonal antibody (STJ29598) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using LDB3 antibody (STJ29598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LDB3 antibody (1-283) (STJ29598)

SKU:
STJ29598

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LDB3 (1-283) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDB3
Gene ID: 11155
Uniprot ID: LDB3_HUMAN
Immunogen Region: 1-283
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human LDB3 (NP_001073585.1).
Immunogen Sequence: MSYSVTLTGPGPWGFRLQGG KDFNMPLTISRITPGSKAAQ SQLSQGDLVVAIDGVNTDTM THLEAQNKIKSASYNLSLTL QKSKRPIPISTTAPPVQTPL PVIPHQKVVVNSPANADYQE RFNPSALKDSALSTHKPIEV KGLGGKATIIHAQYNTPISM YSQDAIMDAIAGQAQAQGSD FSGSLPIKDLAVDSASPVYQ AVIKSQNKPEDEADEWARRS SNLQSRSFRILAQMTGTEF
Tissue Specificity Expressed primarily in skeletal muscle and to a lesser extent in heart. Also detected in brain and placenta.
Function May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
Protein Name Lim Domain-Binding Protein 3
Protein Cypher
Z-Band Alternatively Spliced Pdz-Motif Protein
Cellular Localisation Cytoplasm
Perinuclear Region
Cell Projection
Pseudopodium
Cytoskeleton
Myofibril
Sarcomere
Z Line
Localized To The Cytoplasm Around Nuclei And Pseudopodia Of Undifferentiated Cells And Detected Throughout The Myotubes Of Differentiated Cells
Colocalizes With Actn2 At The Z-Lines
Alternative Antibody Names Anti-Lim Domain-Binding Protein 3 antibody
Anti-Protein Cypher antibody
Anti-Z-Band Alternatively Spliced Pdz-Motif Protein antibody
Anti-LDB3 antibody
Anti-KIAA0613 antibody
Anti-ZASP antibody

Information sourced from Uniprot.org

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