• Immunohistochemistry analysis of LDB1 in paraffin-embedded human colon using LDB1 Rabbit polyclonal antibody (STJ119652) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LDB1 in paraffin-embedded human tonsil using LDB1 Rabbit polyclonal antibody (STJ119652) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LDB1 in paraffin-embedded human uterine cancer using LDB1 Rabbit polyclonal antibody (STJ119652) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using LDB1 Rabbit polyclonal antibody (STJ119652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LDB1 antibody (1-50) (STJ119652)

SKU:
STJ119652

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LDB1 (1-50) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDB1
Gene ID: 8861
Uniprot ID: LDB1_HUMAN
Immunogen Region: 1-50
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-50 of human LDB1 (NP_003884.1).
Immunogen Sequence: MLDRDVGPTPMYPPTYLEPG IGRHTPYGNQTDYRIFELNK RLQNWTEECD
Tissue Specificity Expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Post Translational Modifications Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
Function Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
Protein Name Lim Domain-Binding Protein 1
Ldb-1
Carboxyl-Terminal Lim Domain-Binding Protein 2
Clim-2
Lim Domain-Binding Factor Clim2
Hldb1
Nuclear Lim Interactor
Database Links Reactome: R-HSA-8939236
Reactome: R-HSA-9010553
Reactome: R-HSA-9733709
Reactome: R-HSA-9752946
Cellular Localisation Nucleus
Colocalizes With Slk At Leading Edges
Alternative Antibody Names Anti-Lim Domain-Binding Protein 1 antibody
Anti-Ldb-1 antibody
Anti-Carboxyl-Terminal Lim Domain-Binding Protein 2 antibody
Anti-Clim-2 antibody
Anti-Lim Domain-Binding Factor Clim2 antibody
Anti-Hldb1 antibody
Anti-Nuclear Lim Interactor antibody
Anti-LDB1 antibody
Anti-CLIM2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance