| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-LCP1/plastin-2 (277-291) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000. WB: Approx 70kDa band observed in lysates of cell lines Daudi and U937 (calculated MW of 70.3kDa according to NP_002289.2). Recommended concentration: 0.5-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | LCP1 |
| Gene ID: | 3936 |
| Uniprot ID: | PLSL_HUMAN |
| Immunogen Region: | 277-291 |
| Accession Number: | NP_002289.2 |
| Immunogen Sequence: | HLENAGCNKIGNFST |
| Post Translational Modifications | Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections. |
| Function | Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. |
| Protein Name | Plastin-2 L-Plastin Lc64p Lymphocyte Cytosolic Protein 1 Lcp-1 |
| Database Links | Reactome: R-HSA-8950505 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Junction Cell Projection Ruffle Membrane Peripheral Membrane Protein Cytoplasmic Side Relocalizes To The Immunological Synapse Between Peripheral Blood T-Lymphocytes And Antibody-Presenting Cells In Response To Costimulation Through Tcr/Cd3 And Cd2 Or Cd28 Associated With The Actin Cytoskeleton At Membrane Ruffles Relocalizes To Actin-Rich Cell Projections Upon Serine Phosphorylation |
| Alternative Antibody Names | Anti-Plastin-2 antibody Anti-L-Plastin antibody Anti-Lc64p antibody Anti-Lymphocyte Cytosolic Protein 1 antibody Anti-Lcp-1 antibody Anti-LCP1 antibody Anti-PLS2 antibody |
Information sourced from Uniprot.org
