| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LATS2 (500-550 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Lats2 |
| Gene ID: |
50523 |
| Uniprot ID: |
LATS2_MOUSE |
| Immunogen Region: |
500-550 aa |
| Specificity: |
Specific for LATS2. |
| Immunogen: |
A synthetic peptide from aa region 500-550 of mouse LATS2 conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2. Phosphorylated by MAP4Ks.in parallel to STK3/MST2 and resulting to its activation. Phosphorylation by NUAK2 may regulate its activity in phosphorylation and inactivation YAP1. |
| Function | Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Also phosphorylates YAP1 in response to cell contact inhibition-driven WWP1 ubiquitination of AMOTL2, which results in LATS2 activation. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ. |
| Protein Name | Serine/Threonine-Protein Kinase Lats2Kinase Phosphorylated During Mitosis ProteinLarge Tumor Suppressor Homolog 2Serine/Threonine-Protein Kinase Kpm |
| Database Links | Reactome: R-MMU-2028269 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleNucleusColocalizes With Aurka At The Centrosomes During InterphaseEarly Prophase And CytokinesisMigrates To The Spindle Poles During MitosisAnd To The Midbody During CytokinesisTranslocates To The Nucleus Upon Mitotic Stress By Nocodazole Treatment |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Lats2 antibodyAnti-Kinase Phosphorylated During Mitosis Protein antibodyAnti-Large Tumor Suppressor Homolog 2 antibodyAnti-Serine/Threonine-Protein Kinase Kpm antibodyAnti-Lats2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
