| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Ragulator Complex Protein Lamtor1 (1-161) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LAMTOR1 |
| Gene ID: |
55004 |
| Uniprot ID: |
LTOR1_HUMAN |
| Immunogen Region: |
1-161 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1). |
| Immunogen Sequence: |
MGCCYSSENEDSDQDREERK LLLDPSSPPTKALNGAEPNY HSLPSARTDEQALLSSILAK TASNIIDVSAADSQGMEQHE YMDRARQYSTRLAVLSSSLT HWKKLPPLPSLTSQPHQVLA SEPIPFSDLQQVSRIAAYAY SALSQIRVDAKEELVVQFGI P |
| Post Translational Modifications | N-terminal myristoylation and palmitoylation mediates its recruitment to lysosome membranes, thereby promoting localization of the Ragulator complex to lysosomes. N-myristoylation by NMT1 is required for palmitoylation at Cys-3 and Cys-4. May be palmitoylated by ZDHHC3. Ubiquitinated at Lys-60, Lys-103 and Lys-104 by UBE3A, promoting its degradation by the proteasome. Ubiquitination at Lys-20 impairs the association with the lysosomal V-ATPase complex. Deubiquitination at Lys-20 by USP32 promotes the association with the lysosomal V-ATPase complex and subsequent activation of the mTORC1 complex. |
| Function | Key component of the Ragulator complex, a multiprotein complex involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to the lysosomal membrane. LAMTOR1 wraps around the other subunits of the Ragulator complex to hold them in place and interacts with the Rag GTPases, thereby playing a key role in the recruitment of the mTORC1 complex to lysosomes. Also involved in the control of embryonic stem cells differentiation via non-canonical RagC/RRAGC and RagD/RRAGD activation: together with FLCN, it is necessary to recruit and activate RagC/RRAGC and RagD/RRAGD at the lysosomes, and to induce exit of embryonic stem cells from pluripotency via non-canonical, mTOR-independent TFE3 inactivation. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation. |
| Protein Name | Ragulator Complex Protein Lamtor1Late Endosomal/Lysosomal Adaptor And Mapk And Mtor Activator 1Lipid Raft Adaptor Protein P18Protein Associated With Drms And EndosomesP27kip1-Releasing Factor From RhoaP27rf-Rho |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6798695Reactome: R-HSA-8943724Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013406Reactome: R-HSA-9013407Reactome: R-HSA-9013408Reactome: R-HSA-9013409Reactome: R-HSA-9013423Reactome: R-HSA-9639288 |
| Cellular Localisation | Lysosome MembraneLipid-AnchorCytoplasmic SideLate Endosome MembraneRecruited To Lysosome And Endosome Membranes Through N-Terminal Myristoylation And Palmitoylation |
| Alternative Antibody Names | Anti-Ragulator Complex Protein Lamtor1 antibodyAnti-Late Endosomal/Lysosomal Adaptor And Mapk And Mtor Activator 1 antibodyAnti-Lipid Raft Adaptor Protein P18 antibodyAnti-Protein Associated With Drms And Endosomes antibodyAnti-P27kip1-Releasing Factor From Rhoa antibodyAnti-P27rf-Rho antibodyAnti-LAMTOR1 antibodyAnti-C11orf59 antibodyAnti-PDRO antibodyAnti-PP7157 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and LAMTOR1 knockout (KO) HeLa cells, using [KO Validated] LAMTOR1 Rabbit polyclonal antibody (STJ11100859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89793/358263/STJ11100859_1__20163.1713122603.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LAMTOR1 knockout (KO) HeLa cells, using [KO Validated] LAMTOR1 Rabbit polyclonal antibody (STJ11100859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
