| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LAMP2 (29-410) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000IHC-P 1:100-1:500IF/ICC 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LAMP2 |
| Gene ID: |
3920 |
| Uniprot ID: |
LAMP2_HUMAN |
| Immunogen Region: |
29-410 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 29-410 of human LAMP2 (NP_002285.1). |
| Immunogen Sequence: |
LELNLTDSENATCLYAKWQM NFTVRYETTNKTYKTVTISD HGTVTYNGSICGDDQNGPKI AVQFGPGFSWIANFTKAAST YSIDSVSFSYNTGDNTTFPD AEDKGILTVDELLAIRIPLN DLFRCNSLSTLEKNDVVQHY WDVLVQAFVQNGTVSTNEFL CDKDKTSTVAPTIHTTVPSP TTTPTPKEKPEAGTYSVNNG NDTCLLATMGLQLNITQDKV ASVININPNTTHSTGSCRS |
| Tissue Specificity | Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta. |
| Post Translational Modifications | O- and N-glycosylated.some of the 16 N-linked glycans are polylactosaminoglycans. |
| Function | Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH, NLRP3 and MLLT11, and targeting them for lysosomal degradation. In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2. Plays a role in lysosomal protein degradation in response to starvation. Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation.antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens. Isoform LAMP-2C: Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. |
| Protein Name | Lysosome-Associated Membrane Glycoprotein 2Lamp-2Lysosome-Associated Membrane Protein 2Cd107 Antigen-Like Family Member BLgp-96Cd Antigen Cd107b |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695Reactome: R-HSA-9613829 |
| Cellular Localisation | Lysosome MembraneSingle-Pass Type I Membrane ProteinEndosome MembraneCell MembraneCytoplasmic VesicleAutophagosome MembraneThis Protein Shuttles Between LysosomesEndosomesAnd The Plasma Membrane |
| Alternative Antibody Names | Anti-Lysosome-Associated Membrane Glycoprotein 2 antibodyAnti-Lamp-2 antibodyAnti-Lysosome-Associated Membrane Protein 2 antibodyAnti-Cd107 Antigen-Like Family Member B antibodyAnti-Lgp-96 antibodyAnti-Cd Antigen Cd107b antibodyAnti-LAMP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
