![Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368182/STJ11104366_1__89933.1713133691.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of HeLa cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368183/STJ11104366_2__95394.1713133692.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368184/STJ11104366_3__35038.1713133692.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western blot analysis of lysates from U-937, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368185/STJ11104366_4__39856.1713133693.jpg?c=1 "Western blot analysis of lysates from U-937, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177010/368182/STJ11104366_1__89933.1713133691.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LAMP1 |
| Gene ID: | 3916 |
| Uniprot ID: | LAMP1_HUMAN |
| Immunogen Region: | 29-382 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 29-382 of human LAMP1 (NP_005552.3). |
| Immunogen Sequence: | AMFMVKNGNGTACIMANFSA AFSVNYDTKSGPKNMTFDLP SDATVVLNRSSCGKENTSDP SLVIAFGRGHTLTLNFTRNA TRYSVQLMSFVYNLSDTHLF PNASSKEIKTVESITDIRAD IDKKYRCVSGTQVHMNNVTV TLHDATIQAYLSNSSFSRGE TRCEQDRPSPTTAPPAPPSP SPSPVPKSPSVDKYNVSGTN GTCLLASMGLQLNLTYERKD NTTVTRLLNINPNKTSASG |
| Post Translational Modifications | O- and N-glycosylated.some of the 18 N-linked glycans are polylactosaminoglycans. (Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells. |
| Function | Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Promotes also fusion of the virus with host membrane in less acidic endosomes. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. |
| Protein Name | Lysosome-Associated Membrane Glycoprotein 1 Lamp-1 Lysosome-Associated Membrane Protein 1 Cd107 Antigen-Like Family Member A Cd Antigen Cd107a |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Lysosome Membrane Single-Pass Type I Membrane Protein Endosome Membrane Late Endosome Membrane Cell Membrane Cytolytic Granule Membrane This Protein Shuttles Between Lysosomes Endosomes And The Plasma Membrane Colocalizes With Osbpl1a At The Late Endosome |
| Alternative Antibody Names | Anti-Lysosome-Associated Membrane Glycoprotein 1 antibody Anti-Lamp-1 antibody Anti-Lysosome-Associated Membrane Protein 1 antibody Anti-Cd107 Antigen-Like Family Member A antibody Anti-Cd Antigen Cd107a antibody Anti-LAMP1 antibody |
Information sourced from Uniprot.org